University of Maryland School of Medicine  
PKD Core Center
Antibody Validation/Vector Core Mouse Models BioBank Core
Cell Culture Cell Engineering Core Clinical Translational Core

Core Director
Paul Welling, M.D.

Email:
Phone: (410) 502-4078


Core Co-Director
Owen Woodward, Ph.D.

Email:
Phone: (410) 706-1760


Request Reagents

Protocols

Relevant Commercial Reagents
Ad-CMV-Cre Recombinase
Ad-CMV-Null

The goal of this core is to establish a repository of primary renal epithelial cells and clonal immortalized cell lines derived from genetic animal models relevant to PKD. Our repository will include cell lines from different regions of mouse kidney, specific nephron segments and other tissues. Custom primary cultures and/or immortalized/clonal cell cultures for research base members are available upon request.

Available Cell Models:

1. Pax8rtTA;TetO-Cre;Pkd1cond/cond Immortalized and clonal (beta version)  
2. Pax8rtTA;TetO-Cre;Pkd2cond/cond Immortalized and clonal (beta version)  
3. Pkd1v/v (knock in GPS cleavage mutant) with wild type matched control. Proximal tubule or distal tubule/collecting duct.  
4. Pax8rtTA;TetO-Cre;Pkd1cond/cond;mTmG Primary renal epithelial cells  
5. Pax8rtTA;TetO-Cre;Pkd2cond/cond;mTmG Primary renal epithelial cells  

We will share the beta version cell lines prior to final release with any research base investigator who would like to assist with characterization.

If you receive cell lines from Core D, we ask that you contact Paul Welling with feedback about these reagents.

3D Tubuloid Models:

We can also offer cell material (#4 and #5 above) and protocols for advanced 3D tubuloid modeling of cystogenesis. These methods allow the transition from tubuloids of defined genotype to cysts after the chemical induction of PKD gene loss. Powerful tool for understanding initiating steps in the cell morphological changes underlying cystogenesis in PKD. Please contact Owen Woodward with further questions.

ADPKD Tissue and Primary Cells:

In collaboration with the Clinical Core we offer several resources from our human ADPKD kidney biobank. We have primary cells derived from 100's of unique cysts from human nephrectomized ADPKD kidneys. In addition, we have the corresponding cyst fluid, frozen and fixed samples often all from the same cyst. Our bank includes tissue from both males and females, and a number with characterized germline PKD1 mutations. We offer as controls NHK samples and cells (cortex or medulla) collected from kidneys not suitable for transplant. Please contact Owen Woodward with further questions.

Eduational Programs Pilot & Feasibility Program

The University of Maryland Medical Center and the Division of Nephrology provide multidisciplinary care for patients with PKD. For Appointments call 410-328-5720 and ask for Karleen Schuhart. Make an appointment.

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